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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in lots of tumors, which ends up in chromosome instability, and so might lead to tumorigenesis. This protein was discovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are actually explained. Two further splice variants ended up claimed but detailed nucleotide sequence data is not still readily available.
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Blocking peptides are peptides that bind exclusively into the concentrate on antibody and block antibody binding. These peptide commonly is made up of the epitope acknowledged via the antibody.
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This tutorial outlines further doable optimization of mobile and immunoassay parameters to ensure the very best final results are...
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This guideline outlines even more possible optimization of mobile and immunoassay parameters to make sure the very best effects are...
Antibodies certain to the blocking peptide no longer bind on the epitope around the target protein. By evaluating the staining from your blocked antibody versus the antibody alone, you can see which staining is distinct.
Goal Info Cyclin E belongs towards the really conserved cyclin relatives, whose associates are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins show distinct expression and degradation styles which contribute for the temporal coordination of each mitotic party. Cyclin E types a fancy with and capabilities being a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S changeover. This protein accumulates with the G1-S section boundary and is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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